Parameter

ΔG°37
(kcal/mol)

Symmetrically Equivalent Parameter

5'AA3'
3'UU5'

–0.93 ± 0.03

5'UU3'
3'AA5'

5'AU3'
3'UA5'

–1.10 ± 0.08

  

5'UA3'
3'AU5'

–1.33 ± 0.09

  

5'CU3'
3'GA5'

–2.08 ± 0.06

5'AG3'
3'UC5'

5'CA3'
3'GU5'

–2.11 ± 0.07

5'UG3'
3'AC5'

5'GU3'
3'CA5'

–2.24 ± 0.06

5'AC3'
3'UG5'

5'GA3'
3'CU5'

–2.35 ± 0.06

5'UC3'
3'AG5'

5'CG3'
3'GC5'

–2.36 ± 0.09

  

5'GG3'
3'CC5'

–3.26 ± 0.07

5'CC3'
3'GG5'

5'GC3'
3'CG5'

–3.42 ± 0.08

  

Intermolecular Initiation

+4.09 ± 0.22

  

Per AU end

+0.45 ± 0.04

  

Symmetry correction

+0.43

  

Note that six of the nearest neighbor parameters above have symmetrically equivalent parameters where the bottom strand is placed on top in the 5' to 3' orientation. See, for example, the first example for Watson–Crick helices.