Parameter: |
ΔG°37 loop |
|
| Initiation n = 4 | +1.7 ± 0.51 | |
| Initiation n = 5 | +1.8 ± 0.56 | |
| Initiation n = 6 | +2.0 ± 0.23 | |
| Asymmetry | +0.48 ± 0.04 | |
| AU/GU closure | +0.65 | |
| 1×(n-1) terminal mismatch | 0 | |
| other size terminal mismatch: | GA or AG | –1.1 |
| UU | –0.7 | |
Note: In the context of 1x(n-1) internal loops, i.e. those with a sungle unpaired nucleotide on one side, there are no first mismatch bonuses applied.